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Journal: PLoS ONE
Article Title: The Combinatorial PP1-Binding Consensus Motif (R/K)x (0,1) V/IxFxx(R/K)x(R/K) Is a New Apoptotic Signature
doi: 10.1371/journal.pone.0009981
Figure Lengend Snippet: Cell viability assay : (A) HeLa cells, or (B) other adherent cell lines, were incubated for 24 hours with DPT-AIF peptides prior to processing for MTT staining. In A, SD is shown for n = 3, in B for simplicity the figure corresponds to a representative experiment repeated three times. Cell death assay : (C) HeLa cells were incubated for 2 hrs with DPT-AIF peptides prior to processing for the detection of TUNEL positive cells. The % of positive cells was obtained by ANOVA test and significance was set at P<0.05. (D) To monitor apoptosis in Jurkat cells treated with DPT-AIF peptides, we used Annexin V (left panel) or DiOC 6 (right panel) assays as described in . Statistical analysis was by ANOVA and significance was set at P<0.05.
Article Snippet: Lab-Tek II Chamber Slide w/Cover, 8 well (Ref 154534, Nalge Nunc International), streptavidin Horse Radish Peroxidase conjugate (Euromedex,), 3,3′-diaminobenzydine tetrahydrochloride (DAB) of DAB substrate Kit for Peroxidase (Vector laboratories), “complete mini EDTA free” protease inhibitor cocktail from Roche, O-Phenylenediamine dihydrochloride (OPD) tablet from Sigma chemical, Annexin-V-FITC from Roche, DiOC 6 (3) from Sigma, Kit
Techniques: Viability Assay, Incubation, Staining, TUNEL Assay